Hybridity testing for eggplant hybrids from crosses of drought-tolerant parental lines using SSR markers

Date

12-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Eureka Teresa M. Ocampo

Abstract

This study was conducted to assess the hybridity of first generation progenies resulting from crosses of commercial eggplant varieties Mara and Mistisa with moisture stress-tolerant eggplant accessions PHL 2789 and PHL 4841, respectively. From the polymorphism screening of 65 SSR markers, six markers (EM141, eme05B09, EM133, emh11O01, emf21I02 and EM117) were able to discriminate between Mistisa and PHL 4841 and four markers (CSM20, eme09E09, EM131 and EES063) were able to distinguish Mara form PHL 2789. These markers were then used to determine the hybridity of 30 progenies from each of the respective crosses. For cross Mistisa x PHL 4841, all progenies except for progeny number 13 were identified as hybrids based on the marker data and were selected to proceed to next generation of breeding cycles. Furthermore, all 30 progenies from cross Mara x PHL 2789 showed heterozygosity using the SSR markers and were identified and selected as hybrids.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /M37

Notes

Major in Crop Biotechnology

Document Type

Thesis

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