Molecular cloning and characterization of ribulose-1,5-bisphoshate carboxylase/oxygenase large subunit (rbcL) gene from Pennisetum purpureum Schumach cultivar mott and Pennisetum purpureum Schumach hybrids (Florida and Malaysia)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio C. Laurena

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The study isolated, cloned and characterized the barcoding region of Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) in three genotypes of Napier grass (Pennisetum purpureum Schumach.) namely ?Dwarf', Florida and Malaysia. PCR-based cloning was used to isolate and sequence the barcoding region in rbcL gene. The barcoding region was found to be the first 599 bp of the full rbcL gene. No significant differences were observed in the nucleotide sequences of the amplified rbcL region in the three genotypes of Napier grass studied. In comparison with other Napier grass genotypes, it was found that the rbcL region is highly conserved and minor nucleotide substitutions did not result in any amino acid change upon translation. The amplified rbcL region was most homologous to its relative grass, Cenchrus americanus (Pearl millet) with 99 % homology. A phylogenetic tree based on the 599 bp rbcL barcode sequences from different groups of plants showed the evolutionary relationship of rbcL gene as an acceptable barcoding region. Differences in the rbcL barcode region successfully differentiated selected plants across species and families however, it was not able to discriminate among the three Napier grass genotypes studied.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /M35

Notes

Major in Crop Biotechnology

Document Type

Thesis

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