Total flavonoids production in leaf-derived calli of roselle (Hibiscus sabdariffa L.)

Date

12-2015

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Mylene C. Nieves

Abstract

Callus cultures were successfully established from leaf-derived explants in Murashige and Skoog basal medium with 2.75 mg/L 6-benzylaminopurine (BAP) and 0.175 mg/L indoleacetic acid (IAA). Sucrose at increasing concentrations (3, 5 and 7%), with and without potassium phosphate (KH2PO4) supplementation and exposure to different wavelengths of light (red, white and blue provided by LEDs at 60 µmol/m²/s) were used as elicitors to enhance total flavonoids production in roselle callus cultures. Benzylaminopurine, kinetin and thidiazuron at 0.1, 1.0, and 10 µM concentrations were used for shoot initiation in callus cultures. Flavonoids content in 3% sucrose were comparable to 7% while 5% was the lowest. Sucrose at 7% did not increase callus weight from 7 days onward while 3 and 5% has the same weight for 28 days. Callus cultures in MS medium without potassium phosphate had significantly higher flavonoids content than in complete MS medium but had significantly lower callus weight. Red LEDs had the highest flavonoids content compared with white and blue, but had lower callus weight. Tracheid formation was observed from tissue sections of callus cultures exposed to red LEDs. Calli cultures inoculated onto MS medium amended with different levels of cytokinin (BAP, KIN and TDZ) developed green spots except in 10 µM TDZ at 12 weeks after inoculation. TDZ had the lowest percentage green spot formation but produced high callus growth. Overall findings showed that callusing was promoted instead of shoots in all cytokinins.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2015 A127 /D45

Notes

Major in Crop Biotechnology

Document Type

Thesis

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