Inhibition of meristematic regrowth on Luffa cylindrica L. rootstock and determination of possible DNA transfer between grafted Momordica charantia L. and Luffa cylindrica L.

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Eureka Teresa M. Ocampo

Abstract

Vegetable grafting is becoming more popular with the restriction of soil fumigation to manage soil borne diseases. In addition, grafted plants have increased tolerance to biotic and abiotic stresses and increased plant performance. However there are some issues in using vegetable grafting such as rootstock shoot regrowth that increases the cost of production, and DNA transfer between grafted plants which could change the genetic composition of the more genetically superior scion. It was found out that five chemicals were effectively able to inhibit regrowth of meristem on rootstock without damaging either the rootstock or the scion. Among the chemical treatments, HCl showed the most promising result with only 8.75% of regrowth while also inducing significant increased on growth rate of the treated plants with 6.69 mm/day growth rate compared to the control with 6.12 mm/day growth rate. Following HCl was 2,4-D and silver nitrate with only 8.33% and 10.00% regrowth, respectively, without causing any significant effect on the growth rate of the treated plants. Glyphosate and pendimethalin were also able to inhibit regrowth but in lower percentage with glyphosate having 18.75% and pendimethalin with 20.83% regrowth. Furthermore, treatment with pendimethalin caused a significant decrease in growth rate of the grafted plant with only 3.48 mm/day mean growth rate. There was also no transfer of DNA detected using ISSR marker with primer containing CTC tandem repeats and therefore the grafting induce variation is not due to the transfer of DNA.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /C37

Notes

Major in Crop Biotechnology

Document Type

Thesis

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