Duodenal a-amylase activity of broiler chickens fed diets supplemented with multi-enzyme combination products

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

A 38-day feeding trial was conducted to determine the effects of diets supplemented with multi-enzyme combination products (MECP) on the duodenal α-amylase activity and growth performance of broiler chickens. Three hundred twenty (320) day-old broiler chickens divided and assigned to 4 dietary treatments (T1-Positive Control, T2-Negative Control, T3-Positive+MECP, and T4-Negative+MECP) with 8 replicates per treatment and 10 birds per replicate in a 2 x 2 factorial arrangement completely randomized design (CRD). All growth parameters were determined. At the end of each feeding stage (booster-day 0 to 10, starter- day 11 to 24, finisher- day 25 to 38), 3 birds per treatment were randomly chosen for in vitro α-amylase activity assay. Duodenal digesta were collected and subjected to partial protein purification by salting-out. Protein content determination by Bradford assay and protein profiling via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were conducted prior to α-amylase activity assay using potato starch as substrate. The electrophoretogram of SDS-PAGE showed the qualitative differences in band intensity of α-amylase present in partially purified protein of the duodenal digesta. Results showed no significant interaction and main effects in α-amylase activity on all feeding stages. No significant interaction and main effect were observed in all other growth performance parameters except for interaction effect (P<0.05) on average feed intake at starter stage and main effect (P<0.05) of diets on feed conversion ratio at finisher stage. This study suggests that multi-enzyme combinations supplementation on diets does not positively affect the α-amylase activity and growth performance of broiler chickens in all feeding stages.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /B63

Notes

Major in Animal Biotechnology

Document Type

Thesis

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