Detection of pork contamination in meat products using DNA markers specific to the mitochondrial cytochrome B gene of swine (Sus scrofa)

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Medino Gedeun N. Yebron Jr.

Abstract

This study was conducted to detect pork contamination in meat products using Polymerase chain reaction (PCR)-based methods. PCR was used to detect pig (398bp) DNA in different types of meat (raw, cooked, processed, putretied, halal certified) using published pig-specific primer designed by Matunaga et al. (1999). The specificity of the published pig-specific primers was tested by cross-species amplification test using fourteen different species (pig, coat, cattle, horse, duck, rat, dog, cat, chicken, crocodile, ostrich, sheep, carabao, and human). Results have shown that the tested primers were proven to be pig-specific. PCR was also used to amplify the full length cytochrome b gene of pig (1140bp) using the published novel primers designed by Naidu et al (2012). The PCR fragments were cloned and sequenced. The clones will serve as positive control for the PCR-detection protocol. Using BLAST-N analysis, only one among the seven acquired sequences showed 99% homology to Sus scrofa with an e-value of 0.0. This sequence was used to design a new pig-specific-reverse primer to amplify a shorter PCR product size of 202bp than the 398bp amplicon size of the published primers, which can be used in highly degraded meat samples.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /A78

Notes

Major in Animal Biotechnology

Document Type

Thesis

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