Induction of mutation in sweetpotato (Ipomoea batatas) using diethyl sulfate (DES) and phenotypic and molecular characterization of induced mutants

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Antonio G. Lalusin

Abstract

One thousand eight stem cuttings of NSIC Sp-32 were treated with varying concentrations (3%, 4% and 5%)of Diethyl Sulfate (DES) for 24 and 48 hours. LD50 was established at 5% DES treatment exposed for 48 hours where approximately 50% of the individuals survived. These individuals were considered as putative mutants. A total of 52 putative mutants where characterized morphologically and molecularly. Vine and leaf characteristics showed significant deviation of the putative mutants from the control. Dry matter and starch content results showed significant difference from the control. Band amplifications were observed in 5 out of 11 SSR markers screened. Banding patterns generated by the putative mutants were compared to those generated by the control samples. Results show that 5 SSR markers can already discriminate putative mutants fro the control individuals. Dendogram was constructed for both morphological and molecular data showing varying clusters of the control and putative mutants. Based on the morphological and molecular clustering of 52 putative mutants, 18 individuals were selected and thus can be used as breeding stock for future sweetpotato breeding. However, it is still recommended that these selected individuals should undergo further studies and field trials to validate the results.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /A45

Document Type

Thesis

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