Partial alpha-amylase activity profiling of Aspergillus niger biotech 3080-derived enzymes in rice bran substrate

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Amado A. Angeles

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

To determine the alpha-amylase activity of Aspergillus niger BIOTECH 3080 in rice bran substrate, two (2) fermentation mixtures were incubated for forty-eight (48) hours: T1 (Rice bran D1 fermentation mixture ? Negative Control) and T2 (Rice bran D1 fermentation mixture + A. niger). Isolation of the crude protein from the mixture at 12-hour intervals starting at 0 hour was done by ammonium sulfate (AS) precipitation. Dyalized AS precipitates were analyzed for protein concentration, alpha-amylase activity, and molecular weight. Results showed that the increase in protein concentration of the T2 crude enzyme samples were significantly higher (P<0.05) compared with the negative control. The 0-36 hour protein concentration of T2 was significantly different from each other. Alpha-amylase activity by DNSA method showed considerably higher rate of increase in alpha-amylase activity of T2 compared with the negative control. The 0-36 hour alpha-amylase activity of T2 was significantly different from each other. Alpha-amylase activity at 36-48 hour is at 13.92 umol/ml vs 4.86 umol/ml in negative control. Molecular weight of the alpha-amylase enzyme produced was estimated to be 52kD. Alpha-amylase can be produced from A. niger BIOTECH 3080using rice bran as sole carbohydrate source.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 2016 A127 /A24

Document Type

Thesis

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