Optimization of SDS-PAGE parameters for the isolation of Glutelin in M-19 and M-20 rice varieties and optimization of PCR annealing temperature for SNP2 and W2R molecular markers

Date

6-2016

Degree

Bachelor of Science in Agricultural Biotechnology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Katherine Ann C. Israel

Abstract

The quality of rice is determined by its genes and proteins. The research internship equipped the trainee on the various techniques of protein and DNA analyses. For the first part of the study, proteins were isolated from M-19 and M-20 defatted rice flour varieties provided for by Philippine Rice Research Institute Los Baños. The crude isolate was further separated and characterized via SDS-PAGE to isolate glutelin. The isolated glutelin was subjected to Bradford Assay to determine the protein concentration. The molecular weight of M-19 and M-20 were 6741.989202 Da and 4917.574958 Da, respectively. Furthermore, the second part of the study was conducted at Philippine Rice Research Institute Nueva Ecija to conduct PCR optimization of W2R and SNP2 genes in rice. A PCR gradient was done in order to find the correct annealing temperature of the PCR profiles. Further optimization of the different techniques should be done to be more accurate and precise.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993 2016 A127 /G36

Document Type

Thesis

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