Optimization of growth conditions for the production of Bacteriocin from Pediococcus acidilactici 4E5 using response surface methodology
Date
6-2016
Degree
Bachelor of Science in Chemical Engineering
College
College of Engineering and Agro-Industrial Technology (CEAT)
Adviser/Committee Chair
Jeanne Michelle T. Valencia
Abstract
To enhance production of bacteriocin by Pediococcus acidilactici 4E5, fermentation conditions and medium compositions were optimized using response surface methodology (RSM). Eleven variables were chosen for Plackett-Burman experimental design, in which three variables were screened as the most influencing factors: triammonium citrate, Tween 80 and initial pH of media. The Box-Behnken design was applied to determine a model that accurately describes the effects of the three factors on bacteriocin activity. Analysis of variance (ANOVA) showed a high coefficient of determination value (R^2) of 0.9963, which indicated a good agreement between the experimental and predicted values of bacteriocin activity. The optimum conditions were found to be, 1.075 g/L Tween 80, 1.096 g/L triammonium citrate with and initial pH of 6.871. The validity of the optimal conditions was verified by upscale fermentation using 1.5L of TGYE broth. Under the optimal conditions, an average of 400 AU/mL of pediocin was produced compared to 333 AU/mL before optimization. Also, a decrease of 49.52% on the total cost of a 1 L media was obtained after optimization of the media components. The cost of the bacteriocin is estimated as 0.003 Php/AU.
Language
English
Location
UPLB College of Engineering and Agro-Industrial Technology (CEAT)
Call Number
LG 993.5 2016 E62 /D47
Recommended Citation
Dequito, Meah C., "Optimization of growth conditions for the production of Bacteriocin from Pediococcus acidilactici 4E5 using response surface methodology" (2016). Undergraduate Theses. 5703.
https://www.ukdr.uplb.edu.ph/etd-undergrad/5703
Document Type
Thesis