Optimization of acid hydrolysis of waste biomass from the lipid extraction of Chlorella vulgaris using response surface methodology

Date

6-2016

Degree

Bachelor of Science in Chemical Engineering

Major Course

Major in Sugar Technology

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Monet Concepcion M. Detras

Restrictions

Restricted: Not available to the general public. Access is available only after consultation with author/thesis adviser and only to those bound by the confidentiality agreement.

Abstract

The potential of producing reducing sugars from waste biomass of Chlorella vulgaris by acid hydrolysis was explored in this study. After extracting the lipids from C. vulgaris, the waste biomass contains about 20.41% w/w carbohydrates, which can be converted to reducing sugars. Three factors ? temperature, acid concentration and reaction time ? were initially considered in the 2k factorial analysisto determine which factor significantly affects reducing sugar yield during acid hydrolysis. Results showed that only temperature was found to have insignificant effect on reducing sugar yield, hence, it was not considered in the succeeding optimization step. Both acid concentration and reaction time, on the other hand, were found to have positive direct relationship with reducing sugar yield.Numerical optimization was then done using Response Surface Methodology (RSM) ? Central Composite Diagram to determine the operating conditions for maximum reducing sugar yield. Optimum acid concentration and reaction time were predicted to be about 3.71% v/v and 73.98 min, respectively, resulting to apredicted maximum reducing sugar yield approximately equal to 44.96% w/w. Confirmatory test was done to validate the optimum condition obtained from RSM. There is only 2.50 % error between the predicted and experimental value. With this result as basis, a total of about 35.90% of energy can be theoretically recovered from C. vulgaris by complete conversion of its lipids and reducing sugars to biodiesel and bioethanol, respectively.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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