Kinetics study of gelatin- immobilized Lactococcus lactis BIOTECH K3A22 during batch fermentation using skim milk as substrate

Date

4-2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Marilyn C. Del Barrio

Co-adviser

Susana M. Mercado

Committee Member

Maria Cristina V. Mahilum, Amelia E. Parao, Monet Concepcion C. Maguyon

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Abstract

Different established models were investigated to describe the kinetics of gelatin-immobilized Lactococcus lactis BIOTECH K3A2 during batch fermentation using skim milk as substrate. The pH, titratable acidity and storage life of cell were also determined. The growth curve of gelatin-immobilized Lactococcus lactis BIOTECH K3A2 no lag time and had an exponential phase lasting around four to five hours of the fermentation time. The maximum growth occurred at four to five hours after the introduction the Microorganism. Statistically, there was no significant difference between the growth curves at 8, 10 and 12% skim milk concentrations. Sugar concentration rapidly decrease after the introduction of microorganism suggesting higher density of the cells in milk. The sugar depletion rate at 12% was statistically higher than the other two concentrations. pH and percent titratable acidity showed an indirect relationship wherein low pH resulted in high percent titratable acidity. In assessing the advantage of encapsulated cell for having a long storage life, gelatin cannot be used for long storage since it melted at a faster rate when exposed outside for a long period of time. The Moser, Tessier, and Substrate inhibition models were found not applicable to evaluate the kinetics of fermentation. The Monod equation which tells that the substrate is the only one that limits cell growth provided the best description. The kinetic parameters calculated were Mmx equal 1.9926 per hour and Ks of 49119mg/mL giving R² of 0.9582.

Language

English

LC Subject

Biochemical engineering, Chemical kinetics, Catalysis, Fermentation

Location

UPLB College of Engineering and Agro-Industrial Technology

Call Number

LG 993 2010 E62 E53

Document Type

Thesis

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