Cytological and morphological evaluation of anther culture- derived rice plants from doubled haploid IR43

Date

4-1992

Degree

Bachelor of Science in Agriculture

Major Course

Major in Agronomy

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Edwin L. Javier

Abstract

A total of 21 anther cultured derived-plant from doubled haploid IR43 were cytologically and morphologically evaluated. Thirteen of 21 regenerants were doubled haploids, 7 were putative haploids and 1 was a chimera. The frequency of doubled haploid tended to decrease with increasing age of calli. There was no apparent difference in chromosome behavior observed between the parental first cycle doubled haploids and second cycle doubled haploid plants. Putative second cycle haploid regenerants had a chromosome number ranging from 11 to 24 but PMC with 12I were predominant. Variation in chromosome number could be due to pre-meiotic abnormalities in the regenerant or due to chimeral nature of callus sector that gave rise to the regenerant. Putative second cycle haploids were characterized by absence of regular pairings, non-alignment of some univalent at metaphase I plate, disorganized spindle and unequal distribution of chromosome in anaphase I. The chimeral plant had a chromosome number ranging from 26 to 38, with 36 as the most frequent. Bivalent and univalent formation, unequal distribution of chromosomes and laggards at anaphase I were observed. Putative haploids were sterile and had more tillers than doubled haploids. Second cycle doubled haploids tended to have shorter, narrower, and lighter grains and more tillers than parental first cycle doubled haploids. While the chimeral plant had longer lemma and palea with awns and completely sterile.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

Thesis

Document Type

Thesis

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