Granulation of Milk-Clotting Enzymes (MCE) from Rhizopus chinensis

Date

4-2010

Degree

Bachelor of Science in Chemical Engineering

College

College of Engineering and Agro-Industrial Technology (CEAT)

Adviser/Committee Chair

Marilyn C. Del Barrio

Co-adviser

Susana M. Mercado

Committee Member

Jovita L. Movillon, Jeanne Michelle T. Valencia

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Abstract

The objective of the study was to optimize the operating conditions of producing granules from milk-clotting enzyme produced from Rhizopus chinensis through wet granulation using gelatin as the binder, and lactose and skim milk as the fillers. The factors considered for optimization were amount of gelatin and enzyme, and drying temperature. The response was the milk-clotting activity. Fifteen runs were conducted and Design Expert Statistical Software was used to analyze the data. The optimum points generated were 5 g, 70 mL and 55°C for amount of gelatin, amount of enzyme and drying temperature, respectively. The confirmatory run was done at large scale. The actual activity measured from the granules produced deviated from the predicted by an average of 5.1 percent error for the two trials. The granules were also subjected to particle size distribution analysis. The sauter means and average particle sizes were consistent for the two runs therefore they are reproducible. It was concluded that the amount of enzyme and drying temperature significantly affect the milk-clotting activity while the amount of gelatin does not. Also, the class size and milk-clotting activity were found to be directly related.

Language

English

Location

UPLB College of Engineering and Agro-Industrial Technology

Document Type

Thesis

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