A study on the effect of various culture media on the growth development of Phaleonopsis grandiflora Reich. f. ovules
Date
2023
Degree
Bachelor of Science in Agriculture
Major Course
Major in Agronomy
College
College of Agriculture and Food Science (CAFS)
Adviser/Committee Chair
Helen L. Valmayor
Co-adviser
Grace C. Romero
Abstract
Ovules of Phalaenopsis grandiflora Reichb. f. were excise at the early-embryo and multicellular embryo stage and were cultured on five media-basal Vacin and Went's solution, Vacin and Went's solution plus 25 per cent coconut water, Murashige and Skoog's solution, Knudson's C and Tsuchhiya's medium. Relatively earlier greening was observed in multicellular embryo cultures. Murashige and skoog's medium best enhanced greening and subsequent seedling growth. The addition of coconut water at 25 per cent (by volume) to basal Vacin and Went's solution enhanced greening and multicellular embryos, slighly inhibited seedling growth of early-embryo cultures and seemed to simulate seedling growth of multicellular embryo cultures.
Language
English
Location
UPLB Main Library Special Collections Section (USCS)
Call Number
Thesis
Recommended Citation
Navasero, Rufina C., "A study on the effect of various culture media on the growth development of Phaleonopsis grandiflora Reich. f. ovules" (2023). Undergraduate Theses. 8242.
https://www.ukdr.uplb.edu.ph/etd-undergrad/8242
Document Type
Thesis