An investigation of coliform bacteria in the DTRI-processed soft cheese

Date

3-1983

Degree

Bachelor of Science in Agriculture

Major Course

Major in Animal Science

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Lourdes E. Resubal

Abstract

A study on the coliform bacteria contaminating the soft cheese manufactured on commercial scale at the Dairy Training and Research Institute plant was conducted from April to June, 1982. A total of 27 samples were investigated and the results of the presumptive test showed the occurrence of coliform in all the samples. Likewise, isolation of the cultures were performed on VRB agar, EMB agar and the lactose growth. Results of the tests showed that the cultures isolated closely resemble the coliform bacteria.

The total of 632 reactions to indole, methyl red, Voges-Proskauer and citrate utilization tests of the 158 isolated cultures illustrated 8 distinct IMViC reaction patterns. That was, 48 cultures reacted --/-/- closely related to Aerobacter aerogenes I; 30 isolated cultures we -/--/--- typical of Escherichia coli I; 18 cultures were -/-/-- similar to Citrobacter freundii II; 15 isolated colonies typical of Aerobacter aerogenes II had the IMViC pattern -/- - -/-; cultures were - -/--/- IMViC type closely agreeing with Citrobacter I; 9 cultures were -/- - -/- typical of irregular VII; 7 were ---- IMViC type cultures similar to irregular V and 5 --/-- cultures closely resembled irregular VIII according to the reaction classification proposed by the Coliform Sub-committee (1949), Coli-aerogenes (1956) Sub-committee and by Davis (1963). Fourteen (14) isolated cultures were unclassified. One hundred and fourteen of the isolated cultures were non-fecal in origin while 30 cultures were of fecal origin.

Language

English

Location

UPLB Main Library Special Collections Section (USCS)

Call Number

LG 993.5 1983 A5 A88

Document Type

Thesis

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