Detection and transmission of Papaya ringspot virus biotypes in cucurbit species.

Date

12-2011

Degree

Bachelor of Science in Agriculture

Major Course

Major in Plant Pathology

College

College of Agriculture and Food Science (CAFS)

Adviser/Committee Chair

Sta. Cruz, Filomena C

Abstract

Papaya ringspot virus PRSV has two major biotypes, the PRSV-P and PRSV-W. PRSP-P infects papaya and cucurbits while PRSV-W infects cucurbits but not papaya. The two biotypes are serologically indistinguishable, and their difference can be determined based on their infectivity to both papaya and cucurbits or cucurbits only. The occurrence of natural infection of two PRSV biotypes, PRSV-P and PRSV-W in cucurbit species was determined. Field-grown cucurbit species were tested for PRSV infection through visual assessment of symptom, virus detection and infectivity test. Momordica charantia (bitter gourd) showed mottling and yellowing symptoms but was negative to PRSV in Enzyme-linked Immunosorbent Assay (ELISA). Lugenaria siceraria (bottle gourd), Luffa aegyptica (sponge gourd), Cucurbita maxima (squash) and Diplocyclos palmatus had also virus-like symptoms and were positive to PRSV in ELISA. Virus- infectivity test showed that the virus from field-grown cucurbit crop species were infectious to cucurbit only but not to papaya, indicating that the virus was PRSV-W biotype. However, the virus from field-collected weed species, D. palmatus was infectious to both cucurbit and papaya, indicating that the virus was PRSV-P biotype.

Language

English

Location

Main Library Special Collections Section

Call Number

Thesis

Document Type

Thesis

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