Multiple resistance to medically important antimicrobials of commensal Escherichia coli isolated from dressed broiler chickens in Calabarzon, Philippines

Abstract

© 2018 University of the Philippines at Los Banos. All rights reserved. Commensal Escherichia coli in slaughtered poultry can serve as reservoirs of resistance determinants for humans. The present study aimed to examine the phenotypic resistance of E. coli in chicken cecal samples collected at slaughter from 40 broiler farms in CALABARZON. Whole caeca were taken aseptically from five chicken carcasses randomly selected per farm at the carcass dressing line in four major poultry plants. All birds were determined as healthy by veterinary inspection. E. coli was isolated from the pooled cecal content samples of five birds per farm. A single isolate on each farm was tested for susceptibility by disk diffusion assay to 12 antimicrobials used in human medicine. All isolates were resistant to at least one antimicrobial, presenting a diverse range of resistance phenotypes. Thirty-one resistance patterns and 23 unique profiles were found. Multi-drug resistance (MDR) was observed in 92.5% of isolates. The most common MDR pattern was ampicillin-ciprofloxacin-nalidixic acid-streptomycin-kanamycin-tetracycline (7.5%), mostly against critically important antimicrobials. Highest levels of resistance were observed to nalidixic acid (97.5%), ampicillin (90%), ciprofloxacin (85%), tetracycline (80%), streptomycin (72.5%), trimethoprim (62.5%), and trimethoprim-sulfamethoxazole (62.5 %). Results indicate a very high level of MDR-E. coli in slaughtered broilers to critically important antimicrobials which could be transferred to humans.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

95-106

Document Type

Article

Subject

Antimicrobial resistance, Chicken, E. coli, Philippines

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