Detection of reston ebolavirus from pigs slaughtered in selected abattoirs of Laguna, Philippines using real time RT-PCR

Abstract

© 2018 University of the Philippines at Los Banos. All rights reserved. Detection of Reston ebolavirus (REBOV) was conducted in 384 blood samples from pigs raised and slaughtered in Laguna province which were collected at randomly selected ten (43.5%) out of the total 23 slaughterhouses in Laguna province. The slaughterhouse in Sta. Cruz had the highest number of pigs tested (107, 27.86%), while the lowest number of samples (4, 1.04%) was from the San Pablo slaughterhouse. Females had a slightly higher number, 194 (50.52%) compared to males (190, 49.48%). Blood samples came from pigs from commercial farms registering the highest number of samples at 242 (63.02%), whereas 142 (36.98%) samples came from small-hold farms. In terms of age, 373 (97.14%) were <20 months, 6 (1.56%) animals were aged 41-60 months, and only 5 (1.30%) were aged 21-40 months. The RNA extracted from the sera of these blood samples was subjected to real-time RT-PCR test to detect and profile the occurrence of REBOV. With reference to the sampled population of swine raised and slaughtered in Laguna, no Reston ebolavirus was detected.

Source or Periodical Title

Philippine Journal of Veterinary Medicine

ISSN

317705

Page

141-146

Document Type

Article

Subject

Philippines, Real-time RT-PCR, Reston ebolavirus, Slaughterhouse, Swine

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