Comparative Analysis of Rumen Bacteria between Water Buffalo and Cattle Fed the Same Diet during their Fattening Period in the Philippines

Creator

Ken Asai, Mie University
Khin Ohnmar Lwin, Ministry of Livestock
Abraham G. Tandang, University of the Philippines Los Banos
Rosalina M. Lapitan, University of the Philippines Los Banos
Jesus Rommel V. Herrera, University of the Philippines Los Banos
Arnel N. Del-Barrio, Philippine Carabao Center National Headquarters and Genepool
Makoto Kondo, Mie University
Tomomi Ban-Tokuda, Mie University
Arfan Abrar, Universitas Sriwijaya

Issue Date

2-2021

Abstract

The objective of this study was to compare the differences in ruminal fermentation and the proportion of rumen bacteria between swamp buffalo and cattle in the Philippines. Six crossbred swamp buffalo and six crossbred cattle used in the study were fed the same diet for 16 weeks. Rumen fluid was collected at weeks 0, 8, and 16 of the fattening period. Diversity of the rumen bacteria was analyzed using 454 pyrosequencing. The population density of major cellulolytic bacteria was determined by real-time PCR. Animal performance was not significantly different between buffalo and cattle. The concentration of total short chain fatty acids was found to be higher in the buffalo than in the cattle; however, the proportions of each short chain fatty acid were not significantly different. The proportion of Bacteroidetes increased in buffalo during the sampling period, whereas it was constant in cattle. In contrast, the proportion of Firmicutes and Fibrobacteres decreased in both groups of animals throughout the experiment. Although the density of cellulolytic bacteria between the two groups of animals was not found to be significantly different, the proportion of cellulolytic bacteria decreased during the fattening period. Both animal species exhibited similar traits for composition and population change of bacteria during the fattening period, suggesting that the microbial community structure might be dependent on feed rather than animal species.

Source or Periodical Title

Japan Agricultural Research Quarterly

ISSN

0021-3551

Volume

55

Issue

1

Page

69-75

Document Type

Article

Physical Description

figures, graphs, tables

Language

English

Subject

cattle, pyrosequencing, rumen bacteria, short chain fatty acid, swamp buffalo

Recommended Citation

Asai, K., Lwin, K.O., Tandang, A.G.,Lapitan, R.M., Herrera, J.R.V., Del-Barrio, A.N., Kondo, M., Ban-Tokuda, T., Abrar, A., Fujihara, T., Matsui, H. (2021). Comparative Analysis of Rumen Bacteria between Water Buffalo and Cattle Fed the Same Diet during their Fattening Period in the Philippines. Japan Agricultural Research Quarterly. 55. 69-75. 10.6090/jarq.55.69.

En – AGROVOC descriptors

CATTLE; PYROSEQUENCING; RUMEN BACTERIA; SHORT CHAIN FATTY ACID; SWAMP BUFFALO