Isolation and quantification of hydroxycitric acid from batuan [Garcinia binucao (Blanco) Choisy] fruit

Abstract

© All Rights Reserved. Hydroxycitric acid is a compound with anti-obesity property which is predominant in Garcinia cambogia. In the Philippines, another species, Garcinia binucao, locally known as batuan, is an indigenous fruit used as a souring agent.This study was conducted to determine the most effective method of hydroxycitric acid extraction from batuan (Garcinia binucao) fruit. Acid isolation was performed using three methods including water, methanol and acetone as extraction solvents. Isolated hydroxycitric acid was quantified using spectrophotometric analysis while thin layer chromatography was employed to determine its purity. Results revealed that Garcinia binucao is a potential source of hydroxycitric acid. The extracted hydroxycitric acid from batuan amounted to 4.81 + 0.12 g/100 g sample using water extraction method. Methanol and acetone extraction methods isolated 2.65 + 0.18 g/100 g sample and 2.76 + 0.08 g/100 g sample, respectively. Thin layer chromatography revealed that collected isolate using water extraction method is pure. Water extraction method was found out to be the most effective and efficient method to isolate hydroxycitric acid from batuan fruit.

Source or Periodical Title

International Food Research Journal

ISSN

19854668

Page

706-711

Document Type

Article

Subject

Batuan (Garcinia binucao), Hydroxycitric acid, Solvent extraction, Spectrophotometry, Thin layer chromatography

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