Transmission of episomal Banana streak virus by mealybugs of different host plants

Abstract

© 2017, International Society for Southeast Asian Agricultural Sciences. All rights reserved. The B genome of Musa cultivars contain several infectious endogenous sequences of Banana streak virus (BSV) that constrains bunch growth and harvest. BSV can be easily transmitted into the banana plant by mealybugs (Pseudococcidae) which are known to subsist on banana and other plantain. Several species of mealybugs from different host plants were collected to assess the efficiency to transmit BSV. The study was conducted at the Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños. The results showed that episomal BSV can be transmitted to uninfected banana not only by mealybugs of Musa sp. (Pseudococcus elisae and Dysmicoccus brevipes) but also by mealybugs of Manilkara zapota (unreported), Anonna muricata, Ananas comosus (Dysmicoccus brevipes) and Nephelium napaceum. The mealybugs from pineapple and rambutan had the highest transmission efficiency (100%). The inoculated test plants exhibited typical BSV symptoms at 115 and 267 days post-inoculation when mealybugs from pineapple and rambutan were used respectively. Multiplex immunocapture PCR assay detected four episomal BSV species from the test plants either singly or in mixed infections. Single BSMYV infection elicited the most severe symptoms. Samples that yielded negative results to species identification suggest the presence of new species of BSV in the Philippines.

Source or Periodical Title

Journal of the International Society for Southeast Asian Agricultural Sciences

ISSN

8593132

Page

203-214

Document Type

Article

Subject

Banana streak disease, Immunocapture, Musa, Pseudococcidae, Virus screening

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