Characteristics of four post in vitro-conserved chrysanthemum [Dendranthema grandiflora (Ramat.) kitam.] varieties
Abstract
© 2017, College of Agriculture and Food Science, University of the Philippines Los Banos. All rights reserved. The vegetative and floral characteristics of chrysanthemum [Dendranthema grandiflora (Ramat.) Kitam.] plantlets that had survived after in vitro conservation at different periods and in different media were assessed after growing them in vivo. The surviving plantlets of varieties ‛Pasopati’, ‛Padma Buana’, ‛Puspita Nusantara’ and ‛Tirta Ayuni’ previously conserved under low-temperature conditions in Murashige and Skoog (MS) + 2.5% dimethyl sulfoxide (DMSO), full, ½ and ¼ strengths Tsuchiya media for 2, 4, and 6 mo were acclimatized under protected house conditions with standard cultural maintenance until flowering stage. The initial deviations in plant height, stem diameter, leaf length-width ratio and number of florets within certain culture media and within storage periods were not observed as the storage periods were increased. The size of the flowers, florets and discs, and the color of the adaxial and abaxial parts of all varieties were not affected by the conservation media and length of storage. Cytological alterations in terms of chromosome number, chromosome classification, and grouping were not found within variety and within media. The study showed that in vitro conservation of chrysanthemums is an effective alternative method to replace the more expensive and risky maintenance of live and field-grown germplasm without sacrificing genetic stability.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
317454
Page
358-368
Document Type
Article
Subject
Chrysanthemum (dendranthema grandiflora), Flower disc, In vitro conservation, Karyogram, Tsuchiya medium
Recommended Citation
Budiarto, Kurniawan and Rosario, Teresita L., "Characteristics of four post in vitro-conserved chrysanthemum [Dendranthema grandiflora (Ramat.) kitam.] varieties" (2021). Journal Article. 1254.
https://www.ukdr.uplb.edu.ph/journal-articles/1254