Serological and molecular detection of differential infections of bunchy top and mosaic causing viruses in tissue culture plantlets of Abaca (Musa textilis Née)

Abstract

© 2017, International Society for Southeast Asian Agricultural Sciences. All rights reserved. The abaca planting materials are usually propagated through tissue culture, and its production requires reliable virus indexing protocol to ensure that plants are indeed virus-free. This study aimed to establish a reliable protocol for virus indexing of abaca tissue cultures using serological and molecular methods considering differential infections during the tissue culture process. The objectives were to determine the differential infection of plantlets in tissue culture line and plantlets obtained following subsequent in vitro and ex vitro cultures. The materials were plantlets of in vitro and ex vitro culture lines obtained from infected tissue explant. In this study, differential infection of Banana bunchy top virus (BBTV), Banana bract mosaic virus (BBrMV) and Sugarcane mosaic virus (SCMV) were detected by enzyme-linked immunosorbent assay (ELISA) among plantlets obtained from a single tissue culture line, and in plantlets obtained following subsequent in vitro (first and second subculture) and ex vitro cultures. The differential infection resulted to inconsistency of virus detection by ELISA. Infection of in vitro abaca cultures occurred mostly as mixed infection of two or three viruses. The differential infection of BBTV was confirmed by PCR detection. The sensitivity of BBTV detection by PCR was affected by the dilution of template DNA.

Source or Periodical Title

Journal of the International Society for Southeast Asian Agricultural Sciences

ISSN

8593132

Page

81-93

Document Type

Article

Subject

Bract mosaic, Enzyme-linked immunosorbent assay, Polymerase chain reaction, Sugarcane mosaic, Virus indexing

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