Identification of variety-specific alleles and loci in Philippine citrus collection using simple sequence repeat (SSR) markers
Issue Date
9-2017
Abstract
DNA profiles of 44 citrus varieties from nine species were constructed using 44 polymorphic simple-sequence repeat (SSR) markers to characterize and efficiently identify citrus species and varieties in the Philippines. The SSR-derived similarity coefficients of the collection ranged from 0.43 to 0.77, which was deemed sufficient to delineate the varieties. SSR polymorphism information content (PIC) values were moderate to high, ranging from 0.31 to 0.98, thus proving that SSR is a robust marker for variety identification. Variety-specific markers were identified to systematize and hasten variety identification and authentication. This is the first study using molecular markers to develop an identification key for citrus in the Philippines for faster breeding for commercial purposes.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
0031-7454
Volume
100
Issue
3
Page
296-307
Document Type
Article
Physical Description
illustrations, tables
Language
English
Subject
Citrus, DNA profiles, Polymorphism, SSR markers
Recommended Citation
Tonogbanua, K.A., Espino, R.R.C. (2017). Identification of variety-specific alleles and loci in Philippine citrus collection using simple sequence repeat (SSR) markers. Philippine Agricultural Scientist, 100 (3), 296-307.
Digital Copy
yes
En – AGROVOC descriptors
Citrus, DNA profiles, Polymorphism, SSR markers