Phenotypic characterization and identification of Lysinibacillus sp. NBL1a capable of extracellular biogenic synthesis of gold nanoparticles
Issue Date
7-2017
Abstract
A Gram-positive, rod-shaped bacterial isolate (Isolate NBL1a), isolated from Philippine soil and capable of extracellular biogenic synthesis of gold nanoparticles (AuNPs), was phenotypically-characterized and identified to genus level based on 16s rRNA gene sequencing as Lysinibacillus sp. Extracellular biosynthesis of AuNPs from gold (III) chloride trihydrate (HAuCI4·3H20) by Lysinibacillus sp. NBL1a was achieved using crude cell-free culture supernatants under ambient temperature, without pH adjustments and addition of stabilizers. Visual inspection showed putative gold nanoparticle production in the suspension. UV/Vis scanning spectroscopy of the NBL1a culture supernatant exhibited absorbance peaks characteristic of gold nanoparticles. In the scanning electron microscopy (SEM) of NBL1a, gold nanoparticles were detected to be present with mean size of 50 nm. Energy dispersive X-ray (EDX) analysis further confirmed the AuNPs produced. With these valuable nanoparticles extracellularly produced, there is a great potential for locally-isolated bacteria to be developed as an easy and safe alternative to synthetic methods of production.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
0031-7454
Volume
100
Page
S81-S91
Document Type
Article
Physical Description
illustrations, tables, graphs
Language
English
Subject
Biogenic synthesis, Gold nanoparticles, Green chemistry, Lysinibacillus, Nanobiotechnology
Recommended Citation
Montecillo, A., Carino, S., Ilag, L., Atienza, M.J.T., Fernando, L., Paterno, E.S. (2017). Phenotypic characterization and identification of Lysinibacillus sp. NBL1a capable of extracellular biogenic synthesis of gold nanoparticles. Philippine Agricultural Scientist 100, S81-S91.
Digital Copy
yes
En – AGROVOC descriptors
Biogenic synthesis, Gold nanoparticles, Green chemistry, Lysinibacillus, Nanobiotechnology