Intracellular trafficking modulation by ginsenoside rg3 inhibits brucella abortus uptake and intracellular survival within RAW 264.7 cells

Issue Date

3-2017

Abstract

Ginsenoside Rg3, a saponin extracted from ginseng, has various pharmacological and biological activities; however, its effects against Brucella infection are still unclear. Herein, the inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as immunoblotting and FACS for detecting MAPK signaling proteins and F-actin polymerization, respectively. The internalization, intracellular growth, and adherence of Brucella abortus in Rg3-treated RAW 264.7 cells were significantly decreased compared with the Rg3-untreated control. Furthermore, an apparent reduction of F-actin content and intensity of F-actin fluorescence in Rg3-treated cells was observed compared with B. abortus-infected cells without treatment by flow cytometry analysis and confocal microscopy, respectively. In addition, treating cells with Rg3 decreased the phosphorylation of MAPK signaling proteins such as ERK 1/2 and p38 compared with untreated cells. Moreover, the colocalization of B. abortus-containing phagosomes with LAMP-1 was markedly increased in Rg3-treated cells. These findings suggest that ginsenoside Rg3 inhibits B. abortus infection in mammalian cells and can be used as an alternative approach in the treatment of brucellosis.

Source or Periodical Title

Journal of Microbiology and Biotechnology

ISSN

1017-7825

Volume

27

Issue

3

Page

616-623

Document Type

Article

Language

English

Subject

B. abortus, Ginsenoside Rg3, Inhibitory effect, Intracellular growth, Macrophage

Identifier

doi: 10.4014/jmb.1609.09060.

Digital Copy

yes

Link to Full Text

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