Presence of putative Cycas necrotic stunt virus RNAs in Japanese primrose (Primula sieboldii E. Morren)

Issue Date

12-2016

Abstract

The study screened the putative viral RNA sequences in the cDNA library of Japanese primrose, and conducted a molecular approach in determining its presence in selected Primula sieboldii accessions showing characteristic viral symptoms. Three putatively viral non-homologous sequence groups of RNA were identified; however, coding for different proteins representing a complete virus structure, it was determined to be singly originating from Cycas necrotic stunt virus (CNSV). Subsequently, sequence-specific primers were customised based from the non-homologous-sequence groups; however, amplification data showed no association between the presence of the putative viral RNA sequences and the identified characteristic virus symptoms. Despite this, amplification of the three non-homologous sequences is fully correlated. Thus, Japanese primrose was potentially identified as an alternate host of CNSV.

Source or Periodical Title

Archives of Phytopathology and Plant Protection

ISSN

0323-5408

Volume

50

Issue

3-4

Page

117-122

Document Type

Article

Language

English

Subject

Cycas necrotic stunt virus, flower variegation and leaf variegation, gene expression analysis, Primula sieboldii

Identifier

https://doi.org/10.1080/03235408.2016.1266183

Digital Copy

yes

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