Optimization of raw-starch-digesting amylase (RSDA) production medium for Enterococcus faecium DMF78

Issue Date

2016

Abstract

In an effort to lower the cost of raw-starch-digesting amylase (RSDA) production for E. faecium DMF78, statistical tools were used to optimize production parameters. Plackett-Burman and Box-Behnken has been carried out to evaluate the effects of 17 different parameters and conditions. Proteose peptone, beef extract, and MRS salts were found to be positive significant effectors for amylase production. On the other hand, yeast extract, sodium dodecyl sulfate (SDS), and corn steep liquor (CSL) were negative effectors. The optimized medium resulted to a 466.67% increase in amylase activity and 41.99% decrease in cost compared to the modified MRS medium with the elimination of yeast extract, trub, SDS, and CSL.

Source or Periodical Title

International Food Research Journal

ISSN

1985-4668

Volume

23

Issue

3

Page

1280-1288

Document Type

Article

Physical Description

tables, graphs

Language

English

Subject

Box-Behnken, Enterococcus faecium, Plackett-Burman, Raw-Starch-Digesting Amylase

Digital Copy

yes

Link to Full Text

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