An optimized sustainable medium for the direct lactic acid fermentation of sago (Metroxylon sagu Rottb.) starch by Enterococcus faecium DMF78
Issue Date
2016
Abstract
A low-cost medium for the amylolytic lactic acid bacterium, Enterococcus faecium DMF78, was developed by response surface methodology using sago starch as substrate. Six nitrogen sources, wort, whey, corn steep liquor (CSL), trub, soybean and mungbean flour were evaluated to completely eliminate or dramatically reduce the use of expensive yeast extract, beef extract, and proteose peptone in the medium. Highest lactic acid yield was achieved using a medium with 35.0% whey, 25.0% wort, 3.0% diluted CSL and 0.076% proteose peptone in the standard formulation of de Mann, Rogosa and Sharpe (MRS) medium. Validation run of the optimized medium in the 2-L fermentor revealed slightly lower starch utilization (0.75 g/g) and low cell growth, but high lactic acid yield of 28.83 g/L after 24 hours, as compared to fermentation using complete sago-MRS medium.
Source or Periodical Title
International Food Research Journal
ISSN
1985-4668
Volume
23
Issue
3
Page
1274-1279
Document Type
Article
Physical Description
tables, graphs
Language
English
Subject
Box-Behnken, Enterococcus faecium DMF78, Lactic acid, Nitrogen source alternative, Response surface methodology, Sago starch
Recommended Citation
Toleco, M.R.M., Duque, S.M.M., Tan, A.G., Kobayashi, V.B., Flores, D.M. (2016). An optimized sustainable medium for the direct lactic acid fermentation of sago (Metroxylon sagu Rottb.) starch by Enterococcus faecium DMF78. International Food Research Journal, 23 (3), 1274-1279.
Digital Copy
yes