Simple HPLC method for the analysis of [6]-gingerol produced by multiple shoot culture of ginger (Zingiber officinale)

Abstract

© 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved. A simple HPLC method was developed to analyze [6]-gingerol content of in vitro- grown multiple shoot cultures of ginger. Separation was achieved using an Inertsil ODS-3 column. The best mobile phase was methanol:water (90:10, v/v) which was ran isocratically at a flow rate of 1 ml/min. Analysis of nine different concentrations of [6]-gingerol standards generated a calibration curve which was linear over a concentration range of 1-1000 μg/ml. Limit of detection (LOD) was found to be 0.489 μg/ml, while the limit of quantitation (LOQ) was 1.482 μg/ml. Accuracy of the proposed method was found to be 97.8%. The developed HPLC method was applied to analyze [6]-gingerol production from multiple shoots cultured under different light wavelengths. Results of the analysis revealed that different light wavelengths affected [6]-gingerol production, and that red light irradiation significantly improved [6]-gingerol accumulation in multiple shoot cultures which yielded 191.07±1.25 μg per culture bottle. The developed method is simple, reproducible and is useful for the analysis of [6]-gingerol in multiple shoots of ginger cultured in vitro.

Source or Periodical Title

International Journal of Pharmacognosy and Phytochemical Research

Page

38-42

Document Type

Article

Subject

Ginger, Gingerol, HPLC, Multiple shoot

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