Detection of a novel herpesvirus from bats in the Philippines
Issue Date
5-2015
Abstract
Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to the Retroviridae and Herpesviridae families were identified using next generation sequencing. The existence of herpesvirus in the samples was confirmed by PCR using herpesvirus consensus primers. The nucleotide sequences of the resulting PCR amplicons were 166-bp. Further phylogenetic analysis identified that the virus from which this nucleotide sequence was obtained belonged to the Gammaherpesvirinae subfamily. PCR using primers specific to the nucleotide sequence obtained revealed that the infection rate among the captured bats was 30 %. In this study, we present the partial genome of a novel gammaherpesvirus detected from wild bats. Our observations also indicate that this herpesvirus may be widely distributed in bat populations in Davao region.
Source or Periodical Title
Virus Genes
ISSN
0920-8569
Volume
51
Issue
1
Page
136-139
Document Type
Article
Physical Description
diagram
Language
English
Subject
Bats, Herpesvirus, Next generation sequencing, The Philippines
Recommended Citation
Sano, K., Okazaki, S., Taniguchi, S., Masangkay, J.S., Puentespina, R., Eres, E., Cosico, E., Quibod, M.N.R., Kondo, T., Shimoda, H., Hatta, Y., Mitomo, S., Oba, M., Katayama, Y., Sassa, Y., Furuya, T., Nagai, M., Une, Y., Maeda, K., Mizutani, T. (2015). Detection of a novel herpesvirus from bats in the Philippines. Virus genes, 51 (1), 136-139. doi:10.1007/s11262-015-1197-6.
Identifier
doi:10.1007/s11262-015-1197-6.
Digital Copy
yes