Isolation and partial characterization of a low molecular weight antimicrobial protein from coconut (Cocos nucifera L.) milk

Issue Date

1-2015

Abstract

A low molecular weight protein from coconut (Cocos nucifera L.) milk was extracted using phosphate buffer solution pH 7.6, ammonium sulfate precipitation, and dialysis. The insoluble fraction had higher protein concentration (22.39 μg/mL) than the soluble fraction (9.95 μg/ mL) and the electrophoretic profile on SDS-PAGE also showed that the low molecular weight proteins were concentrated in the insoluble fraction. The fraction containing the low molecular weight proteins were purified using DEAE-Cellulose ion-exchange chromatography followed by gel filtration chromatography using Sephadex G-75. The isolated protein has an estimated molecular weight of 19kDa using the electrophoretic mobility on SDS-PAGE and on the elution parameter by gel filtration chromatography. The low molecular weight protein showed antimicrobial properties against food spoilage yeasts, Debaryomyces hansenii and Candida albicans.

Source or Periodical Title

International Food Research Journal

ISSN

1985-4668

Volume

22

Issue

5

Page

1813-1816

Document Type

Article

Physical Description

illustrations, graphs

Language

English

Subject

Antimicrobial proteins, Coconut proteins, Cocos nucifera L, Low molecular weight, Proteins, SDS-PAGE

Digital Copy

yes

Link to Full Text

Share

COinS