Utilization of sago palm (Metroxylon sagu rottb.) zygotic embryos for cryopreservation
Issue Date
2-2015
Abstract
The value of sago palm (Metroxylon sagu Rottb.) as a high starch yielding crop has not been fully realized yet but at risk to human-mediated and environmental threats that necessitate its conservation. Attempts were made to investigate cryopreservation as an option for long-term storage of sago palm zygotic embryos using simple desiccation technique. Zygotic embryos were aseptically removed from white sago palm seeds. Laminar desiccation (1-3 h) and silica gel drying (1-7 h for 50, 100 and 150 g) were employed to reduce moisture content without losing much viability. Viability was assessed through tetrazolium test and germination in modified Murashige and Skoog media. Desiccation of embryos in 100 g silica gel for 6 h was the selected pre-treatment to cryopreservation. The critical moisture content of embryos was at 21 with 55% viability. With storage in liquid N, staining showed 14-46.7% partial viability after 2 and 24 h, respectively. High desiccation sensitivity of immature sago embryos hindered its direct storage to liquid nitrogen which suggests complementing simple dehydration techniques with cryoprotectants. Cryopreservation of sago palm remains a potential and progressing approach for future long-term conservation and management of this versatile starch crop.
Source or Periodical Title
Asian Journal of Plant Sciences
ISSN
1682-3974
Volume
14
Issue
2
Page
66-71
Document Type
Article
Physical Description
illustrations, tables, graphs
Language
English
Subject
Desiccation, Germination, Laminar, Liquid nitrogen, Silica gel, Tetrazolium test
Recommended Citation
Delima, A.G.D., Damasco, O.P., & Novero, A.U. (2015). Utilization of Sago Palm (Metroxylon sagu Rottb.) Zygotic Embryos for Cryopreservation. Asian Journal of Plant Sciences, 14 (2), 66-71. doi:10.3923/ajps.2015.66.71.
Identifier
doi:10.3923/ajps.2015.66.71.
Digital Copy
yes
