Genetic diversity analysis of eggplant (Solanum melongena L.) and related wild species in the philippines using morphological and SSR markers

Issue Date

12-2014

Abstract

Results of the first genetic diversity assessment of Philippine eggplant germplasm collection consisting of accessions of Solanum melongena L. and related wild species using morphological traits and molecular markers is reported in this paper. Thirty-two accessions, representing 30% of the available collection of local landraces, improved cultivars and crop wild relatives (CWR) held in the national genebank were differentiated based on 39 morphological trait descriptors and 41 Simple Sequence Repeat (SSR) markers. Ten out of the 39 morphological traits accounted for high phenotypic differences among the accessions. SSR polymorphism survey revealed that 33 out of the 41 SSR primer pairs (80.48%) detected variation among the accessions and the number of alleles ranged from 2 to 8 with a mean of 4.3 alleles per marker. The morphological trait and SSR data were analyzed as separate and combined data sets using principal component analysis (PCA) and unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis. Similar results were obtained from the 3 data sets. Landraces, cultivars and crop wild relatives (CWR) were clearly differentiated. However, analysis of all 3 data sets did not distinguish the S. melongena landraces based on geographic areas of collection. CWRs were the most diverse group, followed by the landraces, while the improved cultivars were the least diverse. This study provided significant information for the need to increase the present eggplant collection and to widen the genetic diversity of currently cultivated eggplant varieties in the Philippines.

Source or Periodical Title

Sabrao Journal of Breeding and Genetics

ISSN

1029-7073

Volume

46

Issue

2

Page

183-201

Document Type

Article

Physical Description

illustrations, tables

Language

English

Subject

Eggplant, Genetic diversity, Philippine germplasm, Solanum melongena L, SSR markers, Wild species

Digital Copy

yes

Link to Full Text

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