Degradation of residual Jatropha oil by a promising lipase-producing bacterial consortium

Issue Date

6-2014

Abstract

The potential of using lipase-producing bacteria in degrading the residual oil from Jatropha curcas L. biodiesel wastewater was assessed. Nine bacterial isolates obtained from mangrove areas in the Philippines and seven isolates from biodiesel wastewater were qualitatively and quantitatively screened for lipase activities. The three most promising bacteria (Arthrobacter sp. BOcMJL-12, Bacillus cereus BDF-2 and Pseudoalteromonas sp. BOcMFW-2), with lipase activities of 19.33, 35.67 and 19.50 U/mL, respectively, were selected and evaluated, singly or in combination, for residual Jatropha oil degradation efficiency. The highest oil degradation efficiency of 94.84% was attained using the three isolates in a consortium. The conditions for oil degradation (medium composition, initial medium pH, initial substrate concentration and culture incubation time) by the three bacteria in combination were also partially optimized using the Random Balance Designs. The oil degradation assay, under partially optimized conditions (Medium #5, pH 7 and 1% residual Jatropha oil substrate), revealed that the mixed culture containing the three most promising bacteria could degrade up to 96.99% of residual Jatropha oil after 7-8 d of incubation with continuous agitation. This bacterial consortium may be used as a promising strategy for the treatment of lipid-rich wastes.

Source or Periodical Title

Philippine Journal of Science

ISSN

0031-7683

Volume

143

Issue

1

Page

73-79

Document Type

Article

Physical Description

illustrations, tables

Language

English

Subject

Bacterial consortium, Jatropha, Lipase, Partial optimization, Residual oil

Digital Copy

yes

Link to Full Text

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