Characterization of Virgibacillus sp. BOrMES-11: An anti-Vibrio harveyi strain isolated from Philippine mangrove

Issue Date

6-2014

Abstract

Two hundred mangrove bacteria isolated from soil collected from different mangrove sites in the island of Mindoro were assayed for antimicrobial activity against Vibrio harveyi, the most virulent bacterial pathogen of shrimp in the Philippines. Isolate BOrMES-11 exhibited the highest zone of inhibition (ZOI = 21.0 mm) among four strains that showed antimicrobial activity against the pathogen. This isolate was also found to produce higher extracellular lipase (11.6 U mL-1) and protease (15.8 μg mL-1) at 48 h than at 24 h. Strain BOrMES-11 is a Gram-positive, aerobic, endospore-forming, rod-shaped bacterium that requires at least 1.5% NaCl for growth and is able to grow at salt concentrations of up to 12% NaCl and at pH 10. BLAST analysis of its 16S rRNA gene sequence showed 99% similarity with Virgibacillus dokdonensis DSW-10T and Virgibacillus chiguensis NTU-101T. However, it differs from DSW-10T and NTU-101T in several determinative physiological and biochemical characteristics. Vitek 2 ID System identified the isolate as Bacillus sporothermodurans (90% probability). Rep-PCR fingerprinting showed band differences between BOrMES-11 and V. dokdonensis.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

0031-7454

Volume

97

Issue

2

Page

171-179

Document Type

Article

Physical Description

illustrations, tables, diagram

Language

English

Subject

Anti-vibrio harveyi, Lipase, Mangrove bacteria, Protease

Digital Copy

yes

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