Digital image photometric analysis of Nickel(II) in alkaline solution with dithizone as complexing agent using a mobile phone camera

Issue Date

10-2020

Abstract

Quantitative analysis of nickel(II) in aqueous alkaline solution, with dithizone as a complexing agent, was done using digital image photometry (DIP) and the results were compared with those using conventional visible spectrophotometry (VS). Digital color photographs of prepared standard and sample solutions of Ni(II) were taken using a fabricated lightbox with a 24-well sample holder and iPhone 5S mobile phone camera. RGB values were determined from the photographs using ImageJ software and plots of Red value, L*, a*, and b* as DIP parameters were constructed in order to calculate Ni(II) concentrations. No significant differences in Ni(II) concentrations were observed at a 95% confidence level based on DIP and VS. Performance criteria were set and various tests were conducted for the limit of detection (LOD), limit of quantification (LOQ), linearity/working range, repeatability, and selectivity. The DIP results satisfied the minimal requirements in terms of method validation criteria according to published guidelines. Among the DIP parameters, b* gave results with the highest linearity and accuracy based on the coefficient of determination (R2), as well as plots of least significant differences (LSDs) and linear regression residuals. The results of the present study show that DIP, which uses a relatively inexpensive mobile phone camera, is a promising alternative to the conventional method using an expensive spectrophotometer.

Source or Periodical Title

Philippine Journal of Science

ISSN

0031-7683

Volume

149

Issue

(3-a)

Page

711-717

Document Type

Article

Physical Description

illustrations; graphs; tables; references

Language

English

Subject

Digital photometry, Dithizone reagent, Image processing, Method validation, Ni(II) analysis, RGB

Digital Copy

yes

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