Assessment of genetic diversity and population structure of rice donor germplasm accessions in the Philippines
Issue Date
10-2020
Abstract
Genetic diversity is necessary to safeguard potentially vital traits for adaptation to environmental stresses and a proximate defense against a resurgence of pests and diseases amid climate change. The hybridization core germplasm of the Philippine Rice Research Institute (PhilRice) comprises local and foreign landraces, varieties, and elite breeding lines, which are various sources for diversification and creation of superior rice varieties. The objective of the study is to investigate the level of genetic diversity and population structure across 472 parental germplasm using 17 polymorphic simple sequence repeat (SSR) markers as assistance for rice breeders in the selection of unique parents for hybridization. Germplasm accessions showed high genetic diversity (71%) and formed a structure delineating indica, temperate japonica, and tropical japonica - with 32.8% admixtures identified through the distance-based neighbor-joining (NJ) method, model-based Bayesian structure, and discriminant analysis of principal components (DAPC). Analysis of molecular variance (AMOVA) revealed that the majority of the variation (87.0%) was attributed to accession differences within a population, indicating regular seed movement limited to each subgroup rather than between population (8.7%), wherein the highest fixation index (FST) was observed between tropical and temperate japonica (FST = 0.13). The study established a PhilRice parental germplasm collection containing 306 genetically pure accessions retaining the majority of genetic diversity from the first collection, which offers a promising aid in generating favorable genetic gain through careful genotypic selection in rice breeding programs.
Source or Periodical Title
Philippine Journal of Science
ISSN
0031-7683
Volume
149
Issue
(3-a)
Page
685-696
Document Type
Article
Physical Description
graphs; tables; references
Language
English
Subject
Core collection, Hybridization, Population differentiation, SSR markers
Recommended Citation
Marcelo, V.A.C., Lipio, P.L.G., Labarosa, S.J., Mananghaya, T.E., Niones, J. (2020). Assessment of Genetic Diversity and Population Structure of Rice Donor Germplasm Accessions in the Philippines. Philippine Journal of Science, 149 (3-a), 685-696.
Digital Copy
yes
En – AGROVOC descriptors
CORE COLLECTION; HYBRIDIZATION; POPULATION DIFFERENTIATION; SSR MARKERS