The prevalence of Escherichia coli O157:H7 and other verotoxin-producing E. coli in raw, ground beef samples from wet markets in Laguna, Philippines

Issue Date

6-2013

Abstract

Forty raw, ground beef samples were collected from wet markets in 15 towns of Laguna, Philippines. The samples were enriched using Tryptic Soy Broth (TSB) containing cefixime, cefsulodin and vancomycin and were plated out on Cefixime-Tellurite Sorbitol MacConkey Agar (CT-SMAC). Four hundred presumptive isolates were obtained and screened for typical E. coli O157:H7 characteristics. Two isolates (B2S7-6 and B4S3-6) were determined to be Escherichia coli exhibiting typical E. coli O157:H7 characteristics based on morphological and biochemical tests. Polymerase chain reaction (PCR) using the E. coli DAS™ Kit (BIOTECH) likewise confirmed these isolates to be E. coli. However, none of the isolates was found to possess the O157 antigen using the O157 Latex Agglutination Kit (Oxoid®). The isolates were also tested for genes coding for shiga-like toxin 1 (slt I) and shiga-like toxin 2 (slt II) using PCR and slt I and slt II primers, respectively. Both isolates were negative for slt I but positive for slt II. The prevalence of E. coli O157:H7 in ground beef collected from wet markets in Laguna was 0%. However, the prevalence of non-sorbitol-fermenting, non-ß-glucuronidase-producing E. coli in the collected samples was 5%. The prevalence of verotoxin (Shiga-like toxin)-producing non-O157 E. coli in the beef samples was 5%.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

0031-7454

Volume

96

Issue

2

Page

144-153

Document Type

Article

Physical Description

illustrations, diagrams

Language

English

Subject

Escherichia coli 0157:H7, Ground beef, Verotoxin-producing E. coli

Identifier

doi: 10.1016/j.meatsci.2009.10.011.

Digital Copy

yes

Link to Full Text

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