Conventional isolation and polymerase chain reaction for detection of escherichia coli O157: H7 from intestines of Philippine bats

Issue Date

1-2012

Abstract

It is currently reported that bats in the Philippines harbor bacterial organism (Salmonella spp.) with pathogenic potential. The paper describes the conventional isolation and polymerase chain reaction (PCR) assay for the detection of another bacterium, Escherichia coli, from a sample population of 56 apparently healthy bats collected from Laguna and Quezon City, Philippines. Nineteen of the samples were positive for E. coli using the conventional method of isolation, while PCR molecularly detected the bacteria in 15 samples. The presence of hemolysin among the isolates was not observed. The isolates were subjected to E. coli O157:H7 serotype detection using the latex agglutination test and another PCR assay specific for this serotype. The data revealed that none of the isolates was positive for E. coli O157:H7 using serological and molecular diagnostic methods, which indicates that bats from Laguna and Quezon City, Philippines were not carriers of the pathogenic strain, E. coli O157:H7. The study also presents the first local report of conventional isolation and molecular detection of E. coli from Philippine bats.

Source or Periodical Title

Veterinarski Arhiv

ISSN

0372-5480

Volume

8

Issue

1

Page

283-294

Document Type

Article

Physical Description

tables

Language

English

Subject

Escherichia coli, Latex agglutination test, Philippine bats, Polymerase chain reaction

Digital Copy

yes

Link to Full Text

Share

COinS