Red yeast rice-garlic inhibits the markers of differentiation in 3T3-L1 preadipocytes

Abstract

This study investigates the hypothesis that the ethyl acetate extract of rice fermented with red yeast (Monascuspilosus) in medium containing garlic (RYRGE) inhibits the differentiation of 3T3-L1 preadipocytes by down regulating cellular induction of PPARγ, and C/EBPα. Mouse 3T3-L1 preadipocytes were treated with 0.0, 0.125, and 0.25mg/mL of RYRGE, and Oil Red O staining, triglyceride accumulation and glyceradehyde-3-phosphate dehydrogenase (GPDH) activity were measured as markers of adipocyte differentiation. The protein and mRNA expressions of PPARγ and C/EBPα were measured using Western Blot and RT-PCR, respectively. The mRNA expressions of leptin and aP2 genes were measured using RT-PCR. RYRGE significantly inhibited lipid accumulation and GPDH activity in dose-dependent manner (p<0.05). The mRNA and protein expressions of PPARγ and C/EBPα were dose-dependently inhibited by RYRGE. Similarly, RYRGE decreased the gene expression of adipocyte fatty acid binding protein (aP2) and leptin in a dose-dependent manner (p<0.05), which generally acts as an adipogenic marker proteins and C/EBPα and PPARγ target genes. These results indicate that RYRGE effectively attenuated adipogenesis by down-regulating the expression of adipocyte transcription factors and related adipogenic gene expressions. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Source or Periodical Title

European Journal of Lipid Science and Technology

ISSN

14387697

Page

527-534

Document Type

Article

Subject

3T3-L1 differentiation, AP2, C/EBPα, PPARγ, Red yeast rice-garlic

This document is currently not available here.

Share

COinS