Isolation and characterization of bioactive compounds from seeds of ipil-ipil (Leucaena leucocephala) and its antifeedant activity against the third instar of common cutworm (Spodoptera litura)

Issue Date

9-2020

Abstract

Bioactive compounds from ipil-ipil (Leucaena leucocephala) seeds were isolated using solvent extraction and silica gel column chromatography. Antifeedant activities of the crude ethanolic extract, chromatographic fractions and crystalline isolate were tested on third instar larvae of the common cutworm (Spodoptera litura). Dual choice assay using 10 μg cm-2 applied on both adaxial and abaxial parts of castor (Ricinus communis) leaves showed that the crystalline isolate had the highest activity, with percentage relative feeding inhibition of 79.93 ± 1.00%. The very high melting point, together with data from chemical and spectroscopic analyses, suggest that the isolate is a saponin-containing complex with molar mass of 662 g mol-1 in the major component.

Source or Periodical Title

Philippine Agricultural Scientist, The (Formerly the Philippine Agriculturist)

ISSN

0031-7454

Volume

103

Issue

3

Page

201-206

Document Type

Article

College

College of Agriculture and Food Science (CAFS)

Physical Description

illustration; graph; tables; references

Language

English

Subject

Biopesticides; Bioactive compounds;

Digital Copy

yes

En – AGROVOC descriptors

LEUCAENA LEUCOCEPHALA; SPODOPTERA LITURA; RICINUS COMMUNIS; CHROMATOGRAPHY; SOLVENT EXTRACTION; ANTIFEEDANTS; BIOPESTICIDES; PESTICIDE RESISTANCE; PESTICIDES; PESTICIDAL PROPERTIES; SAPONINS

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