Resistance profile of probiotic lactic acid bacteria against inhibitory effects of selected plant extracts

Issue Date

3-2012

Abstract

Local isolates of bacteriocin-producing lactic acid bacteria (LAB), namely, Pediococcus acidilactici 4E5, P. acidilactici AA5a, P. acidilactici 3G3, P. acidilactici K 3A 2-2, P. pentosaceous K 2A 2-3 and Lactobacillus plantarum BS were subjected to spot-on-lawn antimicrobial assay against aqueous, ethanol and methanol extracts of various plant parts of herbs and vegetables. For comparative purposes, pathogenic Staphylococcus aureus and Salmonella enterica subsp. enterica serovar Typhimurium (formerly known as S. typhimurium) were also used as test microorganisms and three antibiotics (amoxycillin, ampicillin and erythromycin) as antimicrobial agents. Generally, the tested LAB had selective resistance against inhibitory substances of plant part extracts. Methanol extracts were more inhibitory than aqueous and ethanol extracts. The tested pediococci isolates were more affected than the probiotic Lb. plantarum BS, which showed resistance against all plant part extracts including the most potent methanol extract of avocado (Persea americana Mill.) and malunggay (Moringa oleifera Lam.) leaves. All LAB and S. aureus were inhibited by all antibiotics tested, while S. typhimurium was found resistant to erythromycin. Likewise, S. aureus was more sensitive to various plant extracts than S. typhimurium. The antibiotic susceptibility and ability of some LAB to grow on several natural plant extracts suggest the possibility of incorporating these LAB in plant-based nutraceuticals.

Source or Periodical Title

Philippine Agricultural Scientist

ISSN

0031-7454

Volume

95

Issue

1

Page

22-32

Document Type

Article

Physical Description

illustrations, tables, graphs

Language

English

Subject

Antibiotic resistance, Antimicrobial assay, Bacteriocin, Lactic acid bacteria, Nutraceuticals, Plant extracts, Probiotics

Digital Copy

yes

Link to Full Text

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