Mapping QTL for heat tolerance at flowering stage in rice using SNP markers

Issue Date

2011

Abstract

To identify quantitative trait loci (QTL) controlling heat tolerance in rice, the progeny of BC 1F 1 and F 2 populations derived from an IR64×N22 cross were exposed to 38/24°C for 14days at the flowering stage, and spikelet fertility was assessed. A custom 384-plex Illumina GoldenGate genotyping assay was used to genotype the F 2 and selected BC 1F 1 plants. Four single nucleotide polymorphisms were associated with heat tolerance in the BC 1F 1 population using selective genotyping and single marker analysis, and four putative QTL were found to be associated with heat tolerance in the F 2 population. Two major QTL were located on chromosome 1 (qHTSF1.1) and chromosome 4 (qHTSF4.1). These two major QTL could explain 12.6% (qHTSF1.1) and 17.6% (qHTSF4.1) of the variation in spikelet fertility under high temperature. Tolerant allele of qHTSF1.1 was from the susceptible parent IR64, and that of qHTSF4.1 was from tolerant parent N22. The effect of qHTSF4.1 on chromosome 4 was confirmed in selected BC 2F 2 progeny from the same IR64×N22 cross, and the plants with qHTSF4.1 showed significantly higher spikelet fertility than other genotypes. © 2011 Blackwell Verlag GmbH.

Source or Periodical Title

Plant Breeding

ISSN

0179-9541

Volume

131

Issue

1

Page

33-41

Document Type

Article

Language

English

Subject

Flowering stage, Global warming, Heat tolerance, High temperature, Rice, Spikelet fertility

Identifier

DOI:10.1111/j.1439-0523.2011.01924.x

Digital Copy

yes

Link to Full Text

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