Characterization and identification of bacteria in a consortium capable of degrading residual Jatropha biodiesel wastewater oil

Issue Date

1-2012

Abstract

A consortium comprising of three bacteria had been found to have a potential in degrading residual Jatropha oil biodiesel wastewater. Based on a previous study, this promising consortium can degrade up to 97% of residual oil under partially optimized conditions for 8 d. The three bacteria were subjected to phenotypic and genotypic characterizations. Specifically, cultural, morphological, physiological and biochemical testing, and Fatty Acid Methyl Ester (FAME) profiling were conducted. Ribosomal DNA (16S rDNA) sequence analysis was also done. Isolates BOcMFW-2 and BOcMJL-12 were Gram-negative and Gram-positive mangrove bacteria, respectively. On the other hand, Isolate BDF-2 was a Gram-positive bacterium recovered from Jatropha wastewater. These bacteria were identified as: Pseudoalteromonas sp. (BOcMFW-2), Arthrobacter sp. (BOcMJL-12), and Bacillus cereus (BDF-2). The bacterial consortium can be further developed as microbial treatment for biodiesel wastewater.

Source or Periodical Title

Philippine Journal of Science

ISSN

0031-7683

Volume

142

Issue

2

Page

115-124

Document Type

Article

Physical Description

illustrations, tables

Language

English

Subject

Bacteria, Jatropha, Lipase, Phenotypic and genotypic characterizations

Digital Copy

yes

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