Gene for actin is a suitable internal reference for relative RT-PCR-based expression analysis in normal and mutant 'makapuno' endosperms of coconut (Cocos nucifera L.)
Issue Date
7-2012
Abstract
Makapuno is an abnormal coconut with overproliferating endosperm that is softer and fluffy due to accumulation of galactomannan. To normalize the expression of the genes possibly involved in the makapuno phenomenon by semi-quantitative polymerase chain reaction (PCR), an internal control gene was determined. The gene encoding actin exhibited a uniform expression in all the samples studied. In contrast, the expression of the genes coding for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and enolase considerably varied. Therefore, the gene for actin can be used as reference both for normal coconut and mutant makapuno solid endosperm samples at stages 5-6, 6-7 and 7-8 mo.
Source or Periodical Title
Philippine Agricultural Scientist
ISSN
0031-7454
Volume
94
Issue
2
Page
118-123
Document Type
Article
Physical Description
illustrations, tables, graphs
Language
English
Subject
Actin, Coconut endosperm, Endosperm overgrowth, Internal control, Makapuno, Relative RT-PCR
Recommended Citation
Dela Cruz, R.Y., Laude, R.P., Diaz, M.G.Q., Laurena, A.C., Mendioro, M.S., Mendoza, E.M.T. (2011). Gene for Actin is a Suitable Internal Reference for Relative RT-PCRBased Expression Analysis in Normal and Mutant `Makapuno΄ Endosperms of Coconut (Cocos nucifera L.). The Philippine Agriculturist. 94 (2), 118-123.
Digital Copy
yes