Purification, characterization and in vitro cytotoxicity of the bacteriocin from Pediococcus acidilactici K2a2-3 against human colon adenocarcinoma (HT29) and human cervical carcinoma (HeLa) cells
Issue Date
4-2011
Abstract
A bacteriocinogenic lactic acid bacterium (designated K2a2-3) isolated from the intestine of Philippine water buffalo was identified as Pediococcus acidilactici by 16S rRNA gene sequence analysis. The bacteriocin was purified by hydrophobic interaction chromatography, cation-exchange chromatography and reverse phase-high performance liquid chromatography. The purified protein has a molecular mass of 4,625.91 Da, quantified by electrospray ionization time-of-flight mass spectrometry. Based on a BLAST homology search of a partial sequence of 39 amino acid residues and the presence of the structural gene papA, detected through polymerase chain reaction, it was identified as very similar to pediocin PA-1. It was active against a wide spectrum of lactic acid bacteria and Listeria innocua. Partially-purified bacteriocin samples, conducted using pH-mediated bacteriocin extraction method, were found to be cytotoxic against human colon adenocarcinoma (HT29) and human cervical carcinoma (HeLa) cells in vitro, as determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. © 2010 Springer Science+Business Media B.V.
Source or Periodical Title
World Journal of Microbiology and Biotechnology
ISSN
0959-3993
Volume
27
Issue
4
Page
975-980
Document Type
Article
Language
English
Subject
Bacteriocin, Characterization, Cytotoxicity, Pediocin, Pediococcus acidilactici, Purification
Recommended Citation
Villarante, K.I., Elegado, F.B., Iwatani, S., Zendo, T., Sonomoto, K., de Guzman, E.E. (2011). Purification, characterization and in vitro cytotoxicity of the bacteriocin from Pediococcus acidilactici K2a2-3 against human colon adenocarcinoma (HT29) and human cervical carcinoma (HeLa) cells. World Journal of Microbiology and Biotechnology, 27 (4), 975-980. doi:10.1007/s11274-010-0541-1.
Identifier
doi:10.1007/s11274-010-0541-1.
Digital Copy
yes