Influence of 1-methylcyclopropene on pectic enzyme activities, gene expression, and cell wall modification in papaya (Carica papaya cv. 'Sunrise') fruit

Issue Date

12-2011

Abstract

'Sunrise' papaya fruit harvested at two stages of maturity [colour break (< 10% yellow peel colour) and 25% yellow peel colour] were treated with 100 nl l-1 1-methylcyclopropene (1-MCP) to determine its effects on ripening, on the activities and levels of gene expression of polygalacturonase (PG), pectin methyl esterase (PME), and β-galactosidase (β-Gal), and on the degradation of cell wall components. 1-MCP delayed ripening and the onset of the climacteric, although the peak in the respiration rate was almost the same as that in untreated control fruit. Colour-break fruit treated with 1-MCP exhibited a continuous increase in ethylene production, but at a lower rate than in control fruit. Consequently, 1-MCP-treated fruit ripened with a concomitant reduction in firmness, which was accompanied by an increase in PG and β:-Gal enzyme activities and gene expression. On the other hand, fruit treated with 1-MCP at the 25% yellow stage exhibited lower levels of ethylene production and developed pulp with a rubbery texture at the ripe stage which was attributed to reduced PG, β-Gal, and PME enzyme activities and gene expression.This was consistent with the higher level of cell wall polysaccharides measured in 1-MCP-treated fruit. The above results indicated that 'Sunrise' papaya fruit can be treated with 1-MCP at the colour break stage since they have a greater capacity to recover from the effects of 1-MCP than fruit treated at the 25% yellow stage.

Source or Periodical Title

Journal of Horticultural Science and Biotechnology

ISSN

1462-0316

Volume

86

Issue

6

Page

631-639

Document Type

Article

Physical Description

tables, graphs

Language

English

Identifier

doi:10.1080/14620316.2011.11512815.

Digital Copy

yes

Link to Full Text

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