Sequence analyses of the coconut enolase 3' end reveals a CACTG motif found within the 3'- untranslated region

Issue Date

12-2010

Abstract

As the coconut drupe matures, medium-chain fatty acids accumulate suggesting de novo fatty acid synthesis is active until drupe maturation is reached. This suggests that an active transcription of enolase mRNA takes place. Detection of coconut enolase transcripts from 4-5, 5-6, 6-7 and 7-8 month old coconut endosperms were made through 3' RACE. PCR products were cloned and sequence alignment showed higher homology between coconut and palm kernel enolase 3' CDS compared with that of rice and corn. Sequence alignment between the coconut and rice enolase 3' UTR revealed a common CACTG motif which is known to be conserved among the CACTA transposon family and has been speculated to insert regulatory elements. Folding pattern prediction of the coconut enolase 3' UTR revealed the occurrence of folding located immediately after the CACTG motif. We speculate this region to be the binding-site of regulatory elements.

Source or Periodical Title

Philippine Journal of Science

ISSN

0031-7683

Volume

139

Issue

2

Page

227-232

Document Type

Article

Physical Description

illustrations, tables

Language

English

Subject

3'CDS, 3'UTR, Coconut drupe, Coconut endosperm, Enolase, Transposable elements

Digital Copy

yes

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